2 resultados para Microsatellites

em Publishing Network for Geoscientific


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The zip folder comprises a text file and a gzipped tar archive. 1) The text file contains individual genotype data for 90 SNPs, 9 microsatellites and the mitochondrial ND4 gene that were determined in deep-sea hydrothermal vent mussels from the Mid-Atlantic Ridge (genus Bathymodiolus). Mussel specimens are grouped according to the population (pop)/location from which they have been sampled (first column). The remaining columns contain the respective allele/haplotype codes for the different genetic loci (names in the header line). The data file is in CONVERT format and can be directly transformed into different input files for population genetic statistics. 2) The tar archive contains NetCDF files with larval dispersal probabilities for simulated annual larval releases between 1998 and 2007. For each simulated vent location (Menez Gwen, Lucky Strike, Rainbow, Vent 1-10) two NetCDF files are given, one for an assumed pelagic larval duration of 1 year and the other one for an assumed pelagic larval duration of 6 months (6m).

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We sampled leaves from 678 individuals in 21 natural populations (30-36 individuals per population), covering the entire distribution of Euptelea pleiospermum in China.Total DNA was isolated from about 50 mg powdered leaf tissue following the protocol of a DNA extraction kit (Tiangen Biotech Co., LTD., Beijing, China). We used seven fluorescence-labeled microsatellite loci (EP036, EP059, EP081, EP087, EP091, EP278 and EP294; Zhang et al., 2008) to genotype our 678 DNA samples.